By: 17 March 2014
T cells causing transplant rejection involved in pre-term labour

A foetal surgeon and her colleagues have shown, in mice, that pregnancy complications after foetal surgery are triggered by activation of the mother’s T cells – the same T cells that cause the body to reject a donor organ after transplant surgery.

“Preterm labour has been described as the ‘Achilles’ heel’ of the field because it diminishes the benefit of the surgery itself,” said Dr Tippi MacKenzie, an associate professor of surgery and director of research at the UCSF Foetal Treatment Center. “However, specific treatments have not been developed because until now, the biological triggers responsible for preterm birth have been unknown.”

If the same foetal rejection mechanism is occurring in humans, she said, “we have the ability to design specific medical treatments to prevent it – for example, by using medications that target some of the pathways involved in T cell-mediated rejection.” The study was published in the Journal of Immunology.

Normally, pregnancy is a robust form of immune tolerance, in which the pregnant mother naturally tolerates a genetically foreign foetus, MacKenzie explained. “This is in contrast to an organ transplant, where you need to administer immunosuppressive drugs to prevent the body from rejecting a foreign graft. Our study supports the idea that foetal intervention breaks this tolerance by activating the mother’s immune system, suggesting that the biology behind preterm labour is similar to transplant rejection.”

In their study, MacKenzie and her team used a mouse model of foetal intervention to show that after foetal surgery, maternal T cells gather in the uterus. “These are effector T cells, which are the main cells responsible for rejecting a transplanted organ,” said MacKenzie. “In a shift from the normal balance in the uterus, they outnumber regulatory T cells, which are usually responsible for suppressing an immune response against the foetus.”

They next studied genetically modified mice that had T cells designed to recognise and reject one specific foreign protein. They transferred those T cells into the circulation of pregnant mice whose foetuses expressed that protein because they had inherited the gene from their father. The scientists found that, in mice that had foetal surgery, the transferred T cells multiplied and migrated to the uterus.

“It’s known that in a normal pregnancy, T cells that recognise the foetus can circulate in the mother and live in harmony with the foetus,” said first author Marta Wegorzewska, a graduate student in the MacKenzie lab. “But when you perform foetal surgery, they get activated and go to the uterus.”

The researchers then designed an experiment in which half of the pups carried by a pregnant mouse were genetically identical to their mother – as is common among experimental mice – and half were genetically different and expressed foreign proteins inherited from the father. They then injected more of the foreign protein into each foetus in the litter. After this foetal intervention, the scientists observed that there were significantly more deaths among the genetically different pups than among the genetically identical pups.

They then repeated the experiment on a group of mice without T cells and found no difference in the rate of death between the two types of pups.

“This experiment demonstrates that activation of the mother’s T cells after foetal surgery can mediate the death of genetically foreign foetuses,” concluded MacKenzie.

She cautioned that there is a significant difference between her experimental mouse model and human pregnancy: if a mouse pregnancy has complications after foetal surgery, the outcome is not preterm labour but the death of the foetus. “That said, this mouse model is a wonderful tool to study the immune mechanisms of pregnancy complications after surgery,” MacKenzie said.

The next step for her team, she said, “is to determine to what extent foetal interventions trigger the mother’s immune response in humans, or if there is some other cause. Those studies are currently underway.”